Presenting Great Workshops A leadership training lesson from The Pennsylvania State University
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Giving an effective presentation:
Using Powerpoint and structuring a scientific talk
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What do you want from a talk?
Before planning your talk think about its purpose,
the audience you will be talking to, and the setting.
Don’t assume the audience will all be experts.
Never underestimate your audience!
Check on the time that has been allotted to you.
How big is the room?
What do you think of the following slide?
Emk1 knockdown inhibits lumen formation in MDCK cells:
* RT-PCR: EMK1 is effectively knocked down in MDCK cells 24 hours after transfection with P-SUPER (control) or P-SUPER-siEMK1 plasmid; knockdown confirmed on the right with antibodies to EMK1.
* Collagen overlay assay: cells cultured 24 h on collagen I before being overlaid with additional collagen on the apical surface, analyzed 24 h later. Note the lack of lumen in EMK1-KO cultures.
* Ca switch: control or EMK1-KO cells were plated in low Ca medium 24 h upon transfection with pSUPER or pSUPER-KO. After 12 h, cultures were switched to normal medium for 24 h. Transmission EM of cells sectioned perpendicular to the substratum shows lack of microvilli in EMK1-KO cells.
Of course, it is far to confusing and a clear take-home
message does not come across ! (more…)